Hydrogen sulfide induces Ca2+ release from the endoplasmic reticulum and suppresses ATP-induced Ca2+ signaling in rat spinal cord astrocytes

Hydrogen sulfide (H2S) has a variety of physiological functions. H2S reportedly increases intracellular Ca2+ concentration ([Ca2+]i) in astrocytes. However, the precise mechanism and functional role this increase are not known. Here, we examined effects on [Ca2+]i astrocytes from rat spinal cord whether affects ATP-induced signaling, which is known to be involved synaptic function. Na2S (150 ?M), an donor, produced nontoxic [Ca2+]i. The by was inhibited depletion endoplasmic reticulum (ER) but removal extracellular Ca2+, indicating that releases ER. On other hand, when clearly increased astrocytes, suppressed reducing agent. In addition, had no effect cyclic AMP (cAMP) level. These results indicate oxidative post-translational modification proteins cAMP inhibitory signaling. We conclude indirectly inhibits signaling decreasing content ER way, may influence intercellular communication between neurons, thereby contributing neuronal nervous system.